050401Biotech - 1 BIOTECHNOLOGY RECOMBINANT DNA TECHNOLOGY...

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1 BIOTECHNOLOGY BIOTECHNOLOGY RECOMBINANT DNA RECOMBINANT DNA TECHNOLOGY TECHNOLOGY GENETIC ENGINEERING GENETIC ENGINEERING
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2 GENETIC ENGINEERING - RECOMBINANT DNA TECHNOLOGY BIOTECHNOLOGY PURIFICATION OF DNA (Chromosomal and plasmid) RESTRICTION ENZYMES AND CLONING Restriction/Modification Foreign/Self Hemophilus influenzae HincII restriction enzyme Escherichia coli strain R1 EcoRI restriction enzyme Specific sites, palindromes Hybrid molecules constructed in vitro, DNA ligase Vector, Target (or foreign) DNA, Transformation Cloning DNA NUCLEOTIDE SEQUENCE DETERMINATION GENE LIBRARY Eukaryotes, cDNA clone POLYMERASE CHAIN REACTION (PCR) Denature DNA, anneal primers, polymerize with DNA polymerization enzyme, start over Exponential amplification
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3 GENETIC ENGINEERING TECHNIQUES 1. TRANSFORMATION BY PLASMIDS 2. CUTTING DNA AT SPECIFIC SEQUENCES; RESTRICTION ENZYMES CUT SPECIFIC SEQUENCES, CALLED "SITES" RESTRICTION / MODIFICATION NOBEL 3. MOLECULAR CLONING FOREIGN DNA INTO VECTORS GENERATES HYBRID DNA MOLECULES NOBEL 4. DETERMINATION OF DNA SEQUENCE NOBEL 5. SYNTHESIS OF DNA STRANDS OF PREDETERMINED SEQUENCE 6. POLYMERASE CHAIN REACTION- AMPLIFY "ANY" SPECIFIC DNA FRAGMENT OR GENE NOBEL
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4 RESTRICTION / MODIFICATION RESTRICTION - RECOGNITION OF FOREIGN DNA
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This note was uploaded on 02/08/2011 for the course BIO 326R taught by Professor Whiteley during the Spring '08 term at University of Texas.

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050401Biotech - 1 BIOTECHNOLOGY RECOMBINANT DNA TECHNOLOGY...

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