050405Genomics - 1 MICROBIAL GENETICS GENOMICS MICROBIAL...

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1 MICROBIAL GENETICS MICROBIAL GENETICS GENOMICS GENOMICS
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2 MICROBIAL GENETICS - GENOMICS GENOMICS, FUNCTIONAL GENOMICS, STRUCTURAL GENOMICS HOMOLOGY SEQUENCING WHOLE GENOMES Library of Cloned Fragments Contigs Annotation Orthologous, Paralogous GLOBAL STUDIES OF GENE EXPRESSION DNA arrays / Gene arrays / Microarrays / DNA chips Making arrays Labeling RNAs (by making cDNA copies) Hybridization of cDNAs to microarrays CONCEPT OF CORE GENE POOL AND FLEXIBLE GENE POOL Core Gene Pool Replication, Transcription, Translation, Glycolysis, Cell Wall Synthesis Flexible Gene Pool Insertion sequences, transposons, integrons, plasmid, phages (prophages), genomic islands Pathogenicity, Drug Resistance, Toxin Synthesis/Secretion, Conjugation PROTEOMICS
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3 GENOMICS - STUDY OF ALL GENES AND SITES WITHIN THE CHROMOSOME(S) OF AN ORGANISM BY DETERMINING AND ANALYZING THE NUCLEOTIDE SEQUENCE OF THE ENTIRE CHROMOSOME(S) FUNCTIONAL GENOMICS - STUDY OF FUNCTIONS OF A CELL OR ORGANISM BY ANALYSIS OF CHROMOSOME NUCLEOTIDE SEQUENCE STRUCTURAL GENOMICS - STUDY OF 3D SHAPES OF PROTEINS BY COMPARISON OF AMINO ACID SEQUENCES [DEDUCED FROM GENE SEQUENCES] TO PROTEIN STRUCTURE DATA BASES BIOINFORMATICS - DEVELOPMENT OF SOFTWARE AND ALGORITHMS TO ANALYZE GENE AND PROTEIN SEQUENCES AND TO COMPARE THEM TO DATA BASES ALGORITHM - RULES FOR PROCEDURES FOR SOLVING MATHEMATICAL PROBLEM, USUALLY INVOLVING REPETITIVE OPERATIONS HOMOLOGY - EXTENT OF SIMILARITY [OR IDENTITY] BETWEEN GENES OR PROTEINS. PROTEINS WHICH PERFORM THE SAME FUNCTIONS OFTEN HAVE SIMILAR AMINO ACID SEQUENCES. GENES WHICH ENCODE SIMILAR PROTEINS HAVE SIMILAR NUCLEOTIDE SEQUENCES. SOME PROTEINS [AND GENES] ARE VERY SIMILAR AND ARE SAID TO HAVE A HIGH DEGREE OF HOMOLOGY. OTHER PROTEINS AND GENES ARE MORE DISTANTLY RELATED AND SHARE A LOW DEGREE OF HOMOLOGY. UNRELATED PROTEINS HAVE NO SIMILARITY IN SEQUENCE. DNA SEQUENCING - CLONE GENE OR FRAGMENT OF INTEREST, PRIMER,SYNTHESIS OF
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This note was uploaded on 02/08/2011 for the course BIO 326R taught by Professor Whiteley during the Spring '08 term at University of Texas at Austin.

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050405Genomics - 1 MICROBIAL GENETICS GENOMICS MICROBIAL...

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