Lecture4.BioSci97

Lecture4.BioSci97 - Announcements Lecture 4 Exam covers...

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1 Announcements Lecture 4 Exam covers Lectures 1 to 6, and associated problems and readings. Note, not all lecture material is in the powerpoint slides Lectures 5 and 6 will be posted shortly Answers to the recommended odd-numbered problems from Essential Genetics are posted on the web site We will provide the scantron cards The hourly exams from previous years are posted No make-up exams
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2 Central Dogma II: From RNA to Protein Lecture 4 Fall 2009
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3 Sample Questions (Lecture 3) 11. The muscle creatine kinase gene is expressed in muscle cells but not liver cells. Which of the following is the most likely explanation for this tissue- specific gene expression: (A)A muscle-specific transcription factor is found in muscle cells, but not liver cells (B) A muscle-specific enhancer element is found in muscle cells, but not liver cells (C)A muscle-specific 5’ splice junction is found in muscle cells, but not liver cells (D)A muscle-specific rRNA is found in muscle cells, but not liver cells (E) A muscle-specific DNA polymerase is found in muscle cells, but not liver cells 20. Which of the following is true about RNA splicing: (A) The GU-AG rule refers to the exon RNA sequences GU at the 5’ splice site junction and AG at 3’ splice junction (B) Alternative splicing can result in more than one distinct polypeptide chain being produced by a single gene (C)The key component of the spliceosome is the branch point factor (D)The primary RNA transcript does not contain introns (E) Splicing takes place in the cytoplasm
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4 DNA to RNA to Protein
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4-7 Basics of Genetic Code Translation : translate information from RNA (DNA) sequence to protein sequence Universal (nearly) Amino acids are coded for by non-overlapping triplets of nucleotides: codons The “start” codon is AUG and codes for methionine The three “stop” codons terminate translation Open Reading Frame (ORF): Region of mRNA
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Lecture4.BioSci97 - Announcements Lecture 4 Exam covers...

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