Lecture6.BioSci97

Lecture6.BioSci97 - 1 Announcements Lecture 6 Come to the...

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Unformatted text preview: 1 Announcements Lecture 6 Come to the exam knowing your assigned seat (website) and bring #2 pencils, erasers, and student ID Rules of exam 2 Designing PCR Primers They must flank the DNA sequence to be amplified Typically the length of the primer is > 15 bp, but may be smaller Design 6-mer primers to use in a PCR reaction to amplify the DNA sequence shown in red : 5- ATTCGGATTACATCGGCATTACCGATTTAAAGCCCTTAACG-3' 3- TAAGCCTAATGTAGCCGTAATGGCTAAATTTCGGGAATTGC-5' (Please report both your answers in the 5 to 3 orientation) 3 5- ATTCGGATTACATCGGCATTACCGATTTAAAGCCCTTAACG-3 ATTCGG TTAACG 3- TAAGCCTAATGTAGCCGTAATGGCTAAATTTCGGGAATTGC-5' 5- ATTCGGATTACATCGGCATTACCGATTTAAAGCCCTTAACG-3 3- TAAGCCTAATGTAGCCGTAATGGCTAAATTTCGGGAATTGC-5' 5- ATTCGGATTACATCGGCATTACCGATTTAAAGCCCTTAACG-3 ATTCGG TTAACG 3- TAAGCCTAATGTAGCCGTAATGGCTAAATTTCGGGAATTGC-5' 5- ATTCGGATTACATCGGCATTACCGATTTAAAGCCCTTAACG-3 3- TAAGCCTAATGTAGCCGTAATGGCTAAATTTCGGGAATTGC-5' Answer I 5- ATTCGGATTACATCGGCATTACCGATTTAAAGCCCTTAACG-3 3- TAAGCCTAATGTAGCCGTAATGGCTAAATTTCGGGAATTGC-5' 4 5- ATTCGGATTACATCGGCATTACCGATTTAAAGCCCTTAACG-3 AATTGC-5 5-ATTCGG 3- TAAGCCTAATGTAGCCGTAATGGCTAAATTTCGGGAATTGC-5' Answer II 5 Sample Questions (Lecture 5) Mutations in the phenylalanine hydroxylase (PAH) gene in humans can cause a change in a trait. What is the trait? In terms of the central dogma, how does a mutation in the PAH gene cause a change in this trait? 6 Introduction to Meiosis Lecture 6 Bio Sci 97 (B & C) Fall 2009 7 Outline of Lecture 6 Chromosomes Somatic cells versus germ cells and gametes Mitosis Meiosis 8 Chromosomes The genetic material of each cell is divided into separate chromosomes Each eukaryotic chromosome contains a single DNA molecule of enormous length The chromosomes in the nuclei of somatic cells are usually present in pairs. Cells containing two similar sets of chromosomes (genes) are called diploid (2n) Cells containing one set of chromosomes (genes) are haploid (1n) n = number of chromosomes in haploid genome 9 Basic Chromosome Structure During mitosis or meiosis, chromosomes condense and become visible under the microscope Centromere (essential for segregation) Region where spindle fibers attach and move the chromosome during mitosis or meiosis Chromatid One-half of a replicated chromosome Two sister chromatids in replicated chromosome Chromosome 10 Somatic Cells versus Germ Cells and Gametes Somatic cells (2n) form the body of the organism...
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Lecture6.BioSci97 - 1 Announcements Lecture 6 Come to the...

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