hw2_key - CHEM 3511: Survey of Biochemistry Summer 2010...

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‐1‐ CHEM 3511: Survey of Biochemistry Summer 2010 Homework #2: “Essential Biochemistry” Chapters 3 – 5 Due: June 3, 2010 Print Name Roster # 1. Go to the website for New England Biolabs and complete the table below. (5 points each; 30 points) Restriction Enzyme Recognition Sequence Type of Cut (Blunt or Sticky End) Hga I 5’…GACGC(N) 5 …3’ 3’…CTGCG(N) 10 ...5’ Sticky Ends Nco I 5’…C CATGG…3’ 3’…GGTAC C…5’ Sticky Ends Sma I 5’…CCC GGG…3’ 3’…GGG CCC…5’ Blunt 2. Why is the structure of proline considered unique among the 20 standard amino acids? (10 points) Proline is unique due to its cyclic structure; the side chain of proline terminates at the C α amino group creating a closed, cyclic structure. As a result of this rigid conformation, proline is known as a “helix-breaking” residue in protein structure because it reduces the flexibility of polypeptide regions. 3.
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hw2_key - CHEM 3511: Survey of Biochemistry Summer 2010...

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