hw3_key - C HEM 3511 Survey o f B iochemistry S ummer 2010...

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CHEM 3511: Survey of Biochemistry Summer 2010 Homework #3: "Essential Biochemistry" Chapters 6-7 SWER KE Due: June 10. 2010 Roster # Print Name 1. Using Table 6-2, to what enzyme classification does chymotrypsin belong? Hint: Analyze the mechanism of chymotrypsin in Figure 6-1 O. (5 points) HYDROLASE 2. What three amino acids make up the catalytic triad (i.e. the key active site amino acids) in serine proteases such as chymotrypsin? (5 points) SERINE, SER, or S HISTIDINE, HIS, or H ASPARTIC ACID, ASP, or D If only 2 of 3 correct, give 3 points. If only 1 of 3 correct, give 1 point. 3. Look up the following enzymes and fill in the missing information regarding the reaction each one catalyzes. (30 points) ENZYME Converts... Into... Pyruvate kinase Phosphoenolpyruvate + ADP Alcohol + NAO+ J Htl ~T ... Ave \'+1' Pyruvate + ATP Acetaldehyde + NADH + \ Hot (bonus 1pt) "\ Alcohol dehydrogenase Alkaline phosphatase p-Nitrophenylphosphate p-NitTophenol + Phosohate 4. Explain why mutations such as Lys ~ Arg or lie ~ Leu often have little effect on protein function. (10 points) Both
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