Midterm Study Guide-2051

Midterm Study Guide-2051 - Summer 2010 BIOL 2051 General...

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Summer 2010 BIOL 2051 General Microbiology Laboratory Midterm Study Guide There will be four parts to the midterm: Day 1 Section A – you will have to do a streak plate and perform a Gram stain. You will have a total of 25 minutes to do both of these. You must use your time wisely. It is recommended that you do your streak plate while your smear for the Gram stain is air drying. It is not recommended that you do one procedure first and then the other second because you may run out of time before getting done with the second procedure. Day 2 Section B – questions about a set of materials (tubes, plates, etc) on the bench top in front of you. Section C – questions about images you have seen through the microscope. These images will be shown to you by PowerPoint presentation. Section D – short answer questions. I. Stains Stain Structure/ chemical stained Primary stain Mordant Decolorizer Counterstain Result Organisms to remember Simple Basic structure; cellular shape Methylene blue; safranin; CV Air dry and heat fix Water Viewing vasic shapes (p. 34) B. subtilis, S. linguale, B. megaterium, S. coelicolor, D. radiodurans, S. mutans, S. epidermis Gram To understand after Gram staining related to cell wall structure CV Iodine (something that binds to dye) Ethanol Safranin Gram + will be purple and
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Midterm Study Guide-2051 - Summer 2010 BIOL 2051 General...

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