Quiz3Review - Test Results What it Tells you TSI (Triple...

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Unformatted text preview: Test Results What it Tells you TSI (Triple Sugar Iron) RedSlant/RedButt : No fermentation. Peptone aerobically and/or anaerobicaly. (red because peptone used) NOT ENTERIC RedSlant/YellowButt : Glucose fermentation; Peptone catabolized. Glucose fermentation yellow but as glucose used up, revert to peptone breakdown. Cannot overcome acid in butt so still yellow. YellowSlant/YellowButt: Glucose,Lactose, and/or sucrose fermentation. Black stuff : sulfur reduction; breakdown sulfur only when acidic. indicates Fermentation AND sulfur Cracks : Gas made Phenyalanine Deaminase Test (+)Green: FeCl3 added reacts with the phenylpyruvic acid made from removing ammine group off of a.a (-) Yellow Citrate Test (+)Blue: has citrate permease can transport citrate into cell to make pyruvate fermentation. Bromth. Blue turns Basic Blue (-)Green SIM (Sulfide, Indole, Motility) BlackStuff: reduction of thiosulfate (+ indole)RedLayer: tryptophanase , can convert amino acid, tryptophane to indole. Indole reacts with added Kovacs reagent to form rosindole dye which is red in color No Stab: Motile If an organism is motile than the growth will radiate from the stab mark and make the entire tube appear turbid. Stab: NonMotile Decarboxylation Test (+) Purple: Glucose fermentation(yellow) induce decarboxylase alkaline (purple) (-) Yellow: Methyl Red Test QuickTime and a decompressor are needed to see this picture. *Methyl Red added later (+)Red: has buffer to resist pH change, so must be able to produce stable mix acid red (acid) (-)Yellow Vofwa-Peokauer Rwar QuickTime and a decompressor are needed to see this picture. (+)Red: ferment acid acetonin , rct w/ vp reagent red (-) no color change/yellow Urease Test QuickTime and a decompressor are needed to see this picture. (+)Pink : urease fast hydrolyse pink (phenyl red) (-)Yellow (-)orange/uninoculated EMB (Eosin Methylene Blue) Agar Q uickT im e and a decom pressor are needed to see this picture. MetallicGree n: vigorous lactose/sucrose fermentation. Pink: slow lactose/sucrose fermentation Colorless: no lactose/sucrose fermentation API Strip add microbial suspension to each *some add extra suspension to keep aerobic *some add oil for anaerobic Selective Dyes: inhibit gram(+) bacteria Differential Lactose/Sucrose Kirby Bauer Test (Disk Diffusion Assay) Effectivness of antimicrobes against pathogenic microorganisms-when disk soaked with antibiotics placed on...
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This note was uploaded on 02/11/2011 for the course BIOMI 2900 at Cornell University (Engineering School).

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Quiz3Review - Test Results What it Tells you TSI (Triple...

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