Koch's Postulates - Yanting Wang Nov. 10, 2009 Kochs...

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Yanting Wang Nov. 10, 2009 Koch’s Postulate Lab TR 12:20 A.) Postulate #1: It was observed that the infected plant possessed a spiky tumor on the stem, something that the healthy plant lacked, thus implying that the pathogen was in organisms with disease and not in healthy organisms. Postulate #2: A section of the infected plants’ stem tumor was cut off and ground with a drop of EB broth. A section of the healthy plants’ stem was removed and ground as well. Then ground plant materials of the tumor and normal stems were streaked on separate PCA plates and left to incubate at room temperature for a few days. Later, it was noted that the plate streaked from the infected plant contained one type of bacterial colonies, while the plate streaked from the healthy plant material was colony-free. The MASS and CHESST descriptions of the colonies were taken. Thus, fulfilling Postulate 1, shows that the pathogen is only in diseased, and not in healthy. This step also isolated the pathogen and grew it in
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Koch's Postulates - Yanting Wang Nov. 10, 2009 Kochs...

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