bio la 1 - Question How will increasing substrate...

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Question: How will increasing substrate concentration affect the rate (v0) of an enzymatic reaction if the enzyme concentration is held constant for the different trials? Hypothesis: An increase in substrate concentration will increase the rate of an enzymatic reaction until all of the active sites on the enzyme are filled up if the enzyme concentration is held constant for the different trials. Argument in support of the hypothesis: An enzyme is a macromolecule that acts as a catalyst which speeds up a chemical reaction without being consumed by the reaction (Campbell “Biology” 8 th ed. pg 152). An enzyme is never used up in a reaction, and they are always recycled. A substrate is a reactant that the enzyme works on. The enzyme binds to its substrate forming an enzyme-substrate complex. What the enzyme does is that it converts the substrate to a product. The cycle happens so fast that the whole reaction takes place so fast and an enzyme molecule tends to act on about thousands substrate molecules per second. The more substrate
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