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Transformation Lab - 3 What tells you that transformation...

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Transformation lab report: (adapted from the "Carolina" lab sheets) 1. What is the purpose of plating the + and - cells on the LB plates? The purpose of having one with the plasmid and one without the plasmid is it allowed there to be a control within the lab and would ultimately show that having the plasmid should not have any effect on the growth of the ordinary bacteria. 2. What is the purpose of plating the + and - cells on the LB/Amp plates? The purpose of having a plasmid on one AMP plate and not on the other is to show that ordinary bacteria cannot grow on it, but bacteria protected by the plasmid will grow. This is because the AMP is going to kill off the bacteria and there will then be no growth because it will all be gone, but the AMP so therefore can grow.
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Unformatted text preview: 3. What tells you that transformation has been successful and the plasmid is in the recipient cells? The way that I will know that the transformation has been successful is when there is many more colonies, therefore more growth, and also when the colonies will fluoresce. 4. What one plate would you first inspect to conclude that the transformation occurred successfully? Why? The one plate I would inspect to conclude there was transformation would be the plate with the plasmid and the ampicillin, I would look at this one because it has the plasmid which is the subject of transformation and then the ampicillin is what wipes out everything else allowing it to be transformed. 5. Do a transformation efficiency calculation using this document ....
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