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Unformatted text preview: 3. What tells you that transformation has been successful and the plasmid is in the recipient cells? The way that I will know that the transformation has been successful is when there is many more colonies, therefore more growth, and also when the colonies will fluoresce. 4. What one plate would you first inspect to conclude that the transformation occurred successfully? Why? The one plate I would inspect to conclude there was transformation would be the plate with the plasmid and the ampicillin, I would look at this one because it has the plasmid which is the subject of transformation and then the ampicillin is what wipes out everything else allowing it to be transformed. 5. Do a transformation efficiency calculation using this document ....
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- Spring '11