BIS104StarrWinter09Midterm+1+practice[1]

BIS104StarrWinter09Midterm+1+practice[1] - BIS104 Form A...

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BIS104 Form A Midterm I Feb 5, 2010 1 Instructions: There are 9 pages and 30 questions to this exam DO NOT OPEN UNTIL INSTRUCTED. The exam will end promptly at 9:50 am. If you think there is an error in the exam, notify the proctors. The proctors can’t help you define terms or clarify wording. Always pick the best answer. For each question there is one correct answer. Fill out your name and student identification number on the blue Scantron Form with a #2 pencil. Fill in your answers on the scantron form with a #2 pencil. For each question only choose one answer (the best answer) and fill in you selection clearly and completely. If you change an answer, make you sure you erase it completely. There are 30 questions. 20 of them are worth 3 points, and 10 (indicated) are worth 4 points each for a total of 100 possible points.
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BIS104 Form A Midterm I Feb 5, 2010 2 1. You add a KDEL sequence to the C-terminus of cholesterol esterase. Where would you now find cholesterol esterase? A. In the ECM B. In the ER C. In the cytoplasm D. In the Golgi E. In the lysosome 2. You can therefore conclude from question #1 that the KDEL sequence is _______ for the localization of cholesterol esterase to the location in the answer of question #1. A. Necessary B. Sufficient C. Necessary and sufficient D. Neither necessary nor sufficient E. Impossible to conclude 3. BiP is normally a component of the ER lumen. You add a “stop transfer” signal of about 20 hydrophobic amino acids to the middle of the BiP protein sequence. Where would will the C-terminus of this new protein be? A. The ER lumen B. The outside the cell C. The cytoplasm D. The cis-Golgi E. The lysosome 4. (4 points) BiP is normally a component of the ER lumen. You add a “stop transfer” signal of about 20 hydrophobic amino acids to the middle of the BiP protein sequence. Where would you now predict BiP to localize? A. The ER lumen B. Secreted outside the cell C. The ER membrane D. The plasma membrane E. The lysosome 5. (4 points) You isolate a secretion defective mutant strain in yeast that accumulates a great excess of ER derived vesicles. A mutation disrupting which of the following proteins could lead to such a phenotype? A. The GEF protein Sec12 B. The GAP protein in COPII complex C. The G protein ARF1 stuck in the GDP form D. A t-SNARE in the ER membrane E. A COPI complex component
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BIS104 Form A Midterm I Feb 5, 2010 3 6-8. Using sequential differential centrifugation steps, you fractionate cellular organelles. You then run the fractions on an SDS-PAGE gel and detect your favorite proteins (A-E) by western analysis to determine which fraction they are in, resulting in the following results: 6. Where does protein B reside? A. Nucleus
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This note was uploaded on 02/14/2011 for the course BIS 104 taught by Professor Scholey during the Winter '08 term at UC Davis.

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BIS104StarrWinter09Midterm+1+practice[1] - BIS104 Form A...

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