Arinzeh - This is an enhanced PDF from The Journal of Bone...

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The PDF of the article you requested follows this cover page. This is an enhanced PDF from The Journal of Bone and Joint Surgery 2003;85:1927-1935. J Bone Joint Surg Am. Kraus, Alan Smith and Sudha Kadiyala Treena Livingston Arinzeh, Susan J. Peter, Michael P. Archambault, Christian van den Bos, Steve Gordon, Karl Canine Segmental Defect Allogeneic Mesenchymal Stem Cells Regenerate Bone in a Critical-Sized This information is current as of March 20, 2010 Reprints and Permissions Permissions] link. and click on the [Reprints and jbjs.org article, or locate the article citation on to use material from this order reprints or request permission Click here to Publisher Information www.jbjs.org 20 Pickering Street, Needham, MA 02492-3157 The Journal of Bone and Joint Surgery
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COPYRIGHT © 2003 BY THE JOURNAL OF BONE AND JOINT SURGERY, INCORPORATED  Allogeneic Mesenchymal Stem Cells Regenerate Bone in a Critical-Sized Canine Segmental Defect BY TREENA LIVINGSTON ARINZEH, PHD, SUSAN J. PETER, PHD, MICHAEL P. ARCHAMBAULT, MS, CHRISTIAN VAN DEN BOS, PHD, STEVE GORDON, PHD, KARL KRAUS, VMD, ALAN SMITH, PHD, AND SUDHA KADIYALA, PHD Investigation performed at Osiris Therapeutics, Baltimore, Maryland Background: Mesenchymal stem cells from adult bone marrow are multipotent cells capable of forming bone, carti- lage, and other connective tissues. In a previous study, we demonstrated that autologous mesenchymal stem cells could repair a critical-sized bone defect in the dog. The objective of this study was to determine whether the use of al- logeneic mesenchymal stem cells could heal a critical-sized bone defect in the femoral diaphysis in dogs without the use of immunosuppressive therapy. Methods: A critical-sized segmental bone defect, 21 mm in length, was created in the mid-portion of the femoral di- aphysis of twelve adult dogs that weighed between 22 and 25 kg. Each defect was treated with allogeneic mesenchy- mal stem cells loaded onto a hollow ceramic cylinder consisting of hydroxyapatite-tricalcium phosphate. A complete mismatch between donor stem cells and recipient dogs was identified by dog leukocyte antigen typing prior to implan- tation. The healing response was evaluated histologically and radiographically at four, eight, and sixteen weeks after implantation. The radiographic and histological results at sixteen weeks were compared with the historical data for the control defects, which included defects that had been treated with a cylinder loaded with autologous mesenchy- mal stem cells, defects treated with a cylinder without mesenchymal stem cells, and defects that had been left un- treated (empty). The systemic immune response was evaluated by the analysis of recipient serum for production of antibodies against allogeneic cells. Results:
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Arinzeh - This is an enhanced PDF from The Journal of Bone...

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