45 - MCB 142 Professor Georjana Barnes 12/10/07 Lecture 45...

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MCB 142 Professor Georjana Barnes 12/10/07 Lecture 45 ASUC Lecture Notes Online (formerly Black Lightning) is the only authorized note-taking service at UC Berkeley. Please do not share, copy or illegally distribute these notes. Our non-profit, student-run program depends on your individual subscription for its continued existence. These notes are copyrighted by the University of California and are for your personal use only. ANNOUNCEMENTS OH today: 12:30-1:30, 428 Barker. Practice problems - bSpace (don't assume they're exhaustive. If I've forgotten to ask a question about one subject, don't assume that subject won't be on the final. Also, try to review the quizzes your GSIs wrote.) Final - Dec 19, 8-11am, blue gym, DSP: 2030, 2032 VLSB. Monday Discussion sections: yes (today) TA office hours: this week as usual Extra hours to be posted SEMESTER REVIEW OF TOPICS Today, I'm just going to remind you of what we've covered the last five weeks. There's no new material at all today. So we started off considering the mechanisms of mitosis and meiosis and we didn't discuss each stage of mitosis and meiosis, but just the fundamental concepts and the basic terminology of both processes. We discussed what a mutation is and different types of mutations. Then I reminded you what a suppressor is and what it does genetically and how it looks different from a revertant. Then we moved on to gene regulation and we looked at gene regulation at prokaryotes and eukaryotes. We reviewed the basic mechanisms of transcription, initiation, and termination. We introduced the concept and definition of an operon and then we started talking about the concepts of cis and trans in the context of gene regulation. We talked about cis elements, that is, elements that are next to the gene and are physically connected to the gene they are regulating. Trans-acting factors are genes that influence genes from a distance. So we could do cis and trans tests and we did that a lot with the lac operon. Then we talked about constitutive, inducible, and repressible genes and how their regulation differs. Then we looked at specific examples of operons. Then we turned our attention to eukaryotic gene regulation, which is more complicated than in prokaryotes. There are more genes to control and more DNA to regulate. The mechanisms for regulation were much more elaborate in eukaryotes and the reason for that was that there are many cell types in a multi- cellular eukaryote and because many cell types
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45 - MCB 142 Professor Georjana Barnes 12/10/07 Lecture 45...

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