HW1-11_ans - L mole Dissolve 29.22 g of EDTA in about 100...

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Homework #1 1. 8 nanoliters = 8 x 10 -9 L 22 microliters = 2.2 x 10 -5 L 40 milliliters = 4.0 x 10 -2 L 9 deciliters = 9.0 x 10 -1 L 6 kiloliters = 6 x 10 3 L 2. 6.1 mg = 6.1 x 10 -3 g 22 μ g = 2.1 x 10 -5 g 21 mg = 2.1 x 10 -2 g 103 ng = 1.03 x 10 -7 g 10 kg = 1.0 x 10 4 g 3. a 1 mole of Tris x 121.4 g x 0.2 L = 24.28 g of Tris L mole Dissolve 24.28 g of Tris in about 100 ml of H 2 O. Once dissolved, adjust to pH 8 using a pH meter, and then dilute to 200 ml with H 2 O in a graduated cylinder. b 5 moles of NaCl x 58.44 g x 1 L = 292.2 g of NaCl L mole Dissolve 292.2 g of NaCl in about 0.7 L of H 2 O. Once dissolved, dilute to 1 L with H 2 O in a graduated cylinder. c 100 mg of ampicillin x 10 ml = 1 g of ampicillin ml Dissolve 1 g of ampicillin in about 5 ml of H 2 O. Once dissolved, dilute to 10 ml with H 2 O in a graduated cylinder. d 0.5 moles of EDTA x 292.2 g x 0.2 L = 29.22 g of EDTA
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Unformatted text preview: L mole Dissolve 29.22 g of EDTA in about 100 ml of H 2 O. Once dissolved, dilute to 200 ml with H 2 O in a graduated cylinder. e 1 mole of MgCl 2 x 203.3 g x 0.5 L = 101.65 g of MgCl 2 L mole Dissolve 101.65 g of MgCl 2 in about 300 ml of H 2 O. Once dissolved, dilute to 500 ml with H 2 O in a graduated cylinder. 4. For dilutions, use the formula C 1 V 1 =C 2 V 2 . This solution has 2 components: Tris: dilute 1M to 10 mM C 1 V 1 =C 2 V 2 1000 mM x V 1 = 10 mM x 200 ml V 1 = 10 mM x 200 ml 1000 mM = 2 ml of 1 M Tris EDTA: dilute 0.5 M to 1 mM C 1 V 1 =C 2 V 2 500 mM x V 1 = 1 mM x 200 ml V 1 = 1 mM x 200 ml 500 mM = 0.4 ml or 400 L of 0.5 M EDTA Using a 100 ml graduated cylinder, add 2 ml of 1M Tris and 400 L of 0.5 M EDTA. Add water to dilute to a final volume of 200 ml....
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HW1-11_ans - L mole Dissolve 29.22 g of EDTA in about 100...

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