{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

HW1-11_ans

# HW1-11_ans - L mole Dissolve 29.22 g of EDTA in about 100...

This preview shows pages 1–2. Sign up to view the full content.

Homework #1 1. 8 nanoliters = 8 x 10 -9 L 22 microliters = 2.2 x 10 -5 L 40 milliliters = 4.0 x 10 -2 L 9 deciliters = 9.0 x 10 -1 L 6 kiloliters = 6 x 10 3 L 2. 6.1 mg = 6.1 x 10 -3 g 22 μ g = 2.1 x 10 -5 g 21 mg = 2.1 x 10 -2 g 103 ng = 1.03 x 10 -7 g 10 kg = 1.0 x 10 4 g 3. a 1 mole of Tris x 121.4 g x 0.2 L = 24.28 g of Tris L mole Dissolve 24.28 g of Tris in about 100 ml of H 2 O. Once dissolved, adjust to pH 8 using a pH meter, and then dilute to 200 ml with H 2 O in a graduated cylinder. b 5 moles of NaCl x 58.44 g x 1 L = 292.2 g of NaCl L mole Dissolve 292.2 g of NaCl in about 0.7 L of H 2 O. Once dissolved, dilute to 1 L with H 2 O in a graduated cylinder. c 100 mg of ampicillin x 10 ml = 1 g of ampicillin ml Dissolve 1 g of ampicillin in about 5 ml of H 2 O. Once dissolved, dilute to 10 ml with H 2 O in a graduated cylinder. d 0.5 moles of EDTA x 292.2 g x 0.2 L = 29.22 g of EDTA

This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: L mole Dissolve 29.22 g of EDTA in about 100 ml of H 2 O. Once dissolved, dilute to 200 ml with H 2 O in a graduated cylinder. e 1 mole of MgCl 2 x 203.3 g x 0.5 L = 101.65 g of MgCl 2 L mole Dissolve 101.65 g of MgCl 2 in about 300 ml of H 2 O. Once dissolved, dilute to 500 ml with H 2 O in a graduated cylinder. 4. For dilutions, use the formula C 1 V 1 =C 2 V 2 . This solution has 2 components: Tris: dilute 1M to 10 mM C 1 V 1 =C 2 V 2 1000 mM x V 1 = 10 mM x 200 ml V 1 = 10 mM x 200 ml 1000 mM = 2 ml of 1 M Tris EDTA: dilute 0.5 M to 1 mM C 1 V 1 =C 2 V 2 500 mM x V 1 = 1 mM x 200 ml V 1 = 1 mM x 200 ml 500 mM = 0.4 ml or 400 μ L of 0.5 M EDTA Using a 100 ml graduated cylinder, add 2 ml of 1M Tris and 400 μ L of 0.5 M EDTA. Add water to dilute to a final volume of 200 ml....
View Full Document

{[ snackBarMessage ]}