Dr. HJ Yeo – Spring 2011 Homework #2 BCHS 3304 – Spring 2011 Basic Calculations, Amino Acids, Protein Structure and Purification Note: This homework will not be collected. However, exams will assume that you have completed and understand the homework assignment and could answer related questions. Reading Assignment: Chapter 4 of Biochemistry . Chapter 5 of Biochemistry Memorization Assignment: You should be able to draw the structures of each of the twenty amino acids. You should know the 3 letter and 1 letter code for each amino acid. You do NOT have to memorize structures for non-standard amino acids. Show all work and remember to incorporate your units in your calculations. 1 . Your advisor asks you to make two concentrated stock solutions for the whole laboratory to use. She explains that it is common to make a concentrated solution to dilute for use. Thus, a 10x solution refers to one that is 10-fold concentrated. Calculate how you would make 1.0 L of each of the following: A. 10x TBE ( 0.9 M Tris, 0.9 M Boric Acid, 20 mM EDTA)
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