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Unformatted text preview: 2 O. Once the solids are dissolved, dilute the stock solution to 1.0 L with H 2 O in a graduated cylinder to make 50x TAE. 2. TBEdilute 10x to 0.5x C 1 V 1 = C 2 V 2 (10x) x V 1 = (0.5x) x 1.0 L V 1 = (0.5x) x 1.0 L (10x) = 0.05 L or 50 ml of 10x TBE Dilute 50 ml of 10x TBE to 1.0 L with H 2 O in a graduated cylinder to make 0.5x TBE. (Also, you could say 50 ml of 10x TBE plus 950 ml of H 2 O.) 3. TAEdilute 50x to 1x C 1 V 1 = C 2 V 2 (50x) x V 1 = (1x) x 500 ml V 1 = (1x) x 500 ml (50x) = 10 ml of 50x TAE Dilute 10 ml of 50x TAE to 500 ml with H 2 O in a graduated cylinder to make 1x TAE. (Also, you could say 10 ml of 50x TAE and 490 ml of H 2 O.) 4. Methionine Met M 5. Cysteine Cys C...
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This note was uploaded on 02/15/2011 for the course BCHS 3304 taught by Professor Johnson during the Spring '08 term at University of Houston.
 Spring '08
 JOHNSON

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