Lab_7 - Annika Gacnik GSI: Anderson Lo Section 312 Lab 7:...

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Annika Gacnik GSI: Anderson Lo Section 312 Lab 7: Thin Layer Chromotography Discussion To determine the two TLC solvents most useful in part II, we looked at the data from part I. We used four different solvents, 60:40 hexane:ethyl acetate, 100% ethyl acetate, 92:8 ethyl acetate:acetic acid, and 10:10:1 toluene:ethyl acetate:acetic acid on four TLC plates. Each plate tracked the movement of the same four compounds, aspirin, acetaminophen, ibuprofen, and caffeine. We looked at how separated the spots of the compounds were from each other after the TLC plate was saturated with solvent and exposed under UV light. If a majority of the spots remained closer to the baseline than the solvent front, the solvent was not polar enough and the compounds could not separate. They would then have similar R f in that solvent system and would be difficult to differentiate if we did not already know their identities. If a majority of the spots moved closer to the solvent front than the baseline, the solvent was too polar and the same
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This note was uploaded on 02/15/2011 for the course CHEM 3A taught by Professor Fretchet during the Fall '08 term at Berkeley.

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Lab_7 - Annika Gacnik GSI: Anderson Lo Section 312 Lab 7:...

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