Biosensors3 - ELECTROCHEMICAL BIOSENSORS Modern and future...

Info icon This preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
ELECTROCHEMICAL BIOSENSORS Modern and future approaches to medical diagnostics James F. Rusling Dept. of Chemistry, Univ CT, Storrs, Dept. of Cell Biology, Univ. of CT Health Center F. A. Armstrong, H. A. Heering, and J. Hirst, Reactions of complex metalloproteins studies by protein film voltammetry. J. Chem. Soc. Rev . 26 , 169-179 (1997). J. F. Rusling, Z. Zhang, Designing functional biomolecular films on electrodes. in J. F. Rusling, Ed., Biomolecular Films , Marcel Dekker, N. Y., 2003 , pp. 1-64.
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Medical Diagnostics Doctors increasingly rely on testing Needs: rapid, cheap, and “low tech” Done by technicians or patients Some needs for in-vivo operation, with feedback
Image of page 2
electrode substrate product Figure 9 Enzyme (label) Apply voltage Measure current prop. to concentration of substrate Principle of Electrochemical Biosensors
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
E, V time E-t waveform potentiostat Electrochemical cell counter working electrode N 2 inlet Protein film reference insulator electrode material Equipment for developing electrochemical biosensors Cyclic voltammetry
Image of page 4
Electrode enzyme A lipid-enzyme film
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
-2 -1.5 -1 -0.5 0 0.5 1 1.5 2 -0.8 -0.6 -0.4 -0.2 0 0.2 0.4 0.6 0.8 I , μ A E, V vs SCE Cyclic voltammogram (CV) at 100 mV s -1 and 25 o C of Mycobacterium Tuberculosis KatG catalase-peroxidase in a thin film of dimyristoylphosphatidylcholine on basal plane PG electrode, in anaerobic pH 6.0 buffer. Oxidation Of Fe II Reduction Of Fe III Reversible Peaks for Direct electron Transfer (not all proteins do this)
Image of page 6
Catalytic enzyme electrochemistry a basis for biosensor - glucose oxidase oxidation Fc mediator Fc + glucose + enzyme I = f [glucose] A. Cass, G. Davis, G. D. Francis, H. O. A. Hill, W. J. Aston, I. J. Higgins, E. V. Plotkin, L. D. L. Scott, A. P. F. Turner, Anal. Chem. 56, 667-671 (1984). Mediator shuttles Electrons between Enzyme and electrode
Image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Scheme 2 Glucose + GO(FAD) + 2 H + ! gluconolactone + GO(FADH 2 ) (1) GO(FADH 2 ) + 2 Fc + ! GO(FAD) + 2 Fc + 2 H + (4) Fc ! Fc + + 2 e - (at electrode) (5) Mechanism for catalytic oxidation of glucose With Glucose oxidase (GO) and Fc mediator Signal can also be measured by amperometry: Hold const. E where oxidation occurs, measure I vs time Fc = ferrocenecarboxylate
Image of page 8
Commercial Glucose Sensors Biggest biosensor success story!
Image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern