Biosensors3

Biosensors3 - ELECTROCHEMICAL BIOSENSORS Modern and future...

Info iconThis preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
ELECTROCHEMICAL BIOSENSORS Modern and future approaches to medical diagnostics James F. Rusling Dept. of Chemistry, Univ CT, Storrs, Dept. of Cell Biology, Univ. of CT Health Center F. A. Armstrong, H. A. Heering, and J. Hirst, Reactions of complex metalloproteins studies by protein film voltammetry. J. Chem. Soc. Rev . 26 , 169-179 (1997). J. F. Rusling, Z. Zhang, Designing functional biomolecular films on electrodes. in J. F. Rusling, Ed., Biomolecular Films , Marcel Dekker, N. Y., 2003 , pp. 1-64.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Medical Diagnostics Doctors increasingly rely on testing Needs: rapid, cheap, and “low tech” Done by technicians or patients Some needs for in-vivo operation, with feedback
Background image of page 2
electrode substrate product Figure 9 Enzyme (label) Apply voltage Measure current prop. to concentration of substrate Principle of Electrochemical Biosensors
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
E, V time E-t waveform potentiostat Electrochemical cell counter working electrode N 2 inlet Protein flm re±erence insulator electrode material Equipment for developing electrochemical biosensors Cyclic voltammetry
Background image of page 4
Electrode enzyme A lipid-enzyme flm
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
-2 -1.5 -1 -0.5 0 0.5 1 1.5 2 -0.8 -0.6 -0.4 -0.2 0 0.2 0.4 0.6 0.8 I , μ A E, V vs SCE Cyclic voltammogram (CV) at 100 mV s -1 and 25 o C of Mycobacterium Tuberculosis KatG catalase-peroxidase in a thin film of dimyristoylphosphatidylcholine on basal plane PG electrode, in anaerobic pH 6.0 buffer. Oxidation Of Fe II Reduction Of Fe III Reversible Peaks for Direct electron Transfer (not all proteins do this)
Background image of page 6
Catalytic enzyme electrochemistry a basis for biosensor - glucose oxidase oxidation Fc mediator Fc + glucose + enzyme I = f [glucose] A. Cass, G. Davis, G. D. Francis, H. O. A. Hill, W. J. Aston, I. J. Higgins, E. V. Plotkin, L. D. L. Scott, A. P. F. Turner, Anal. Chem. 56, 667-671 (1984). Mediator shuttles Electrons between Enzyme and electrode
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Scheme 2 Glucose + GO(FAD) + 2 H + ! gluconolactone + GO(FADH 2 ) (1) GO(FADH 2 ) + 2 Fc + ! GO(FAD) + 2 Fc + 2 H + (4) Fc ! Fc + + 2 e - (at electrode) (5) Mechanism for catalytic oxidation of glucose With Glucose oxidase (GO) and Fc mediator Signal can also be measured by amperometry: Hold const. E where oxidation occurs, measure I vs time Fc = ferrocenecarboxylate
Background image of page 8
Commercial Glucose Sensors Biggest biosensor success story!
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 02/15/2011 for the course CMPD 201 taught by Professor Loo during the Spring '11 term at Maryland.

Page1 / 32

Biosensors3 - ELECTROCHEMICAL BIOSENSORS Modern and future...

This preview shows document pages 1 - 10. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online