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tmvslides - Tobacco mosaic virus - - TMV PLANT VIROLOGY 100...

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Tobacco mosaic virus --TMV
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PLANT VIROLOGY – 100 years of TMV Virus replication & gene expression Movement protein identified & function determined Resistance gene cloned ( N -gene) Plants engineered with anti-virus resistance Father of Virology Father of Virology •1 st virus (1898 - Beijerinck) • Crystallized (1935 - Stanley) • Nucleoprotein (1936) • Assembled in vitro (1955) • RNA infectious (1956) • X-ray structure (1977) TMV Firsts TMV Firsts TMV is a model virus used for many pioneering studies: TMV is a model virus used for many pioneering studies: Scholthof K-B et al., 1999 APS pubilcation
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Wendell Stanley – 1935 • At Rockefeller Foundation in Princeton • Crystallized TMV, received the Nobel prize • Began molecular biology at UC Berkeley Stanley Hall, U.C. Berkeley TMV Morris presents Morris presents seminar at Stanley seminar at Stanley Hall Hall – circa 1973 circa 1973
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Experimental Mechanical Transmission of Plant Viruses
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Diatomite is a sedimentary rock containing a high percentage of fossil diatoms, a single-celled type of algae that commonly lives at the surfaces of oceans or lakes. These diatoms sink, collecting on the bottom at very slow rates of a cm/1,000 years. CELITE, The Abrasive
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Mechanical inoculation of plant viruses. Buffer, abrasive. Wounds are temporary and heal within minutes.
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TMV infections in tobacco. At left shows necrotic local lesions resulting from a hypersensitive response when tobacco contains the resistance gene N . At right show tobacco leaves exhibiting mosaic symptoms typical of systemic infection.
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COMPOSITION OF TMV VIRIONS TMV virions are rod shaped, 300nm long and about 18nm in diameter. The virions have helical symmetry and a hollow, cylindrical core. Virion mass is 40 million RNA is 2 million, the protein capsid is 38 million. How can a genome of 2 million encode for a 38 million MW protein?
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2 million MW RNA = 6,400 nucleotides 6,400 nucleotides divided by 3 (one codon) = 2133 codons. 2133 X 110 (avg. MW of an amino acid) = 234,630!!! Far less than the 38 million MW capsid!!! What is the coding capacity of the TMV genomic RNA?
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COMPOSITION OF TMV VIRIONS TMV virions are rod shaped, 300nm long and about 18nm in diameter. The virions have helical symmetry and a hollow, cylindrical core.
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This note was uploaded on 02/16/2011 for the course MIC 162 taught by Professor Manning during the Spring '09 term at UC Davis.

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tmvslides - Tobacco mosaic virus - - TMV PLANT VIROLOGY 100...

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