LAB REPORT - Wong Benny Wong Bio 101 section 5DF2 Friday...

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Wong Benny Wong Bio 101 section 5DF2 Friday session Lab Abstract There were three purposes to this lab: to analyze the effect of changing enzyme concentration on the rate of reaction, how various pHs affect the rate of activity of amylase, and how various temperatures affect the rate of activity of amylase. Enzymes are proteins that speed up chemical reactions by binding the enzyme and the substrate together. The substrate is then modified into the product. After the enzyme and product dissociate, the enzyme is ready to be used again for another reaction. Amylase was the enzyme analyzed in this lab. It is used to break down starch (a complex sugar) into a simple sugar. Throughout all the experiments the rate of enzymatic activity was measured through the use of a test plate with well drops along with an I 2 KI solution. The results show that in experiment A, the there is a direct relationship between enzyme concentration and reaction rate. In experiment B, there is an optimal pH (between the pH of 6 or 7) at which amylase activity occurs. In experiment C, the results show that there is an optimal temperature (between 22 ° C and 37 ° C) at which amylase activity occurs. Introduction Enzymes are proteins that catalyze chemical reactions. In reactions that involve enzymes, the molecules at the beginning of the reactions are called substrates. At the end of the enzymatic reaction, the substrate is modified to form the product. The active site of an enzyme is selective for their substrates and is also where the reaction occurs. The enzyme and substrate bind together to form the enzyme-substrate complex. After catalysis of the reaction occurs, the enzyme and the product(s) dissociate and the enzyme is unchanged and is available for re-use. An enzyme 1
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Wong accelerates the rate of reactions by lowering the activation energy required for a reaction to take place. Many factors contribute to how well an enzyme works, such as pH and temperature. Activators are organic or inorganic molecules in the cell that squeeze into the active site of an
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This note was uploaded on 02/16/2011 for the course BIO 101 taught by Professor Kaplan during the Spring '11 term at CUNY City.

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LAB REPORT - Wong Benny Wong Bio 101 section 5DF2 Friday...

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