{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

LAB REPORT - Wong Benny Wong Bio 101 section 5DF2 Friday...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Wong Benny Wong Bio 101 section 5DF2 Friday session Lab Abstract There were three purposes to this lab: to analyze the effect of changing enzyme concentration on the rate of reaction, how various pHs affect the rate of activity of amylase, and how various temperatures affect the rate of activity of amylase. Enzymes are proteins that speed up chemical reactions by binding the enzyme and the substrate together. The substrate is then modified into the product. After the enzyme and product dissociate, the enzyme is ready to be used again for another reaction. Amylase was the enzyme analyzed in this lab. It is used to break down starch (a complex sugar) into a simple sugar. Throughout all the experiments the rate of enzymatic activity was measured through the use of a test plate with well drops along with an I 2 KI solution. The results show that in experiment A, the there is a direct relationship between enzyme concentration and reaction rate. In experiment B, there is an optimal pH (between the pH of 6 or 7) at which amylase activity occurs. In experiment C, the results show that there is an optimal temperature (between 22 ° C and 37 ° C) at which amylase activity occurs. Introduction Enzymes are proteins that catalyze chemical reactions. In reactions that involve enzymes, the molecules at the beginning of the reactions are called substrates. At the end of the enzymatic reaction, the substrate is modified to form the product. The active site of an enzyme is selective for their substrates and is also where the reaction occurs. The enzyme and substrate bind together to form the enzyme-substrate complex. After catalysis of the reaction occurs, the enzyme and the product(s) dissociate and the enzyme is unchanged and is available for re-use. An enzyme 1
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Wong accelerates the rate of reactions by lowering the activation energy required for a reaction to take place. Many factors contribute to how well an enzyme works, such as pH and temperature.
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern