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Spectrophotometric Determination of ManganeseAdam CapriolaNovember 28, 2006CHM 1112 Section 109 Unknown Sample: 14IntroductionA spectrophotometer measures the amount of light absorbed by a solution at different wavelengths of light emitted. Beer’s Law says that absorbance is equal to molar absorptivity times the thickness of the sample times the concentration of the sample. Beer’s law also states that conformity of a solution is able to be determined by plotting its absorbances versus its concentrations, and if a straight line results crossing through the origin, the solution has conformity. Using this information, it is possible to determine an unknown concentration of a solution by finding its absorbance, or if given its concentration, its absorbance can be found without the use of a spectrophotometer.ExperimentalFirst, a spectrophotometer was turned on, allowed to warm up for about 15 minutes, and was set at a wavelength 400 nm. A cuvette filled with deionized water was used for blanking the spectrophotometer. A second cuvette was filled with a solution of potassium permanganate which was provided. Each cuvette was wiped with a Kimwipe before being placed in the spectrophotometer in order to eliminate smudges which could affect the light passing through.

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