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SCAN0002 - BIOREFINERY CONCEPT(“urn Grain Si I‘m In...

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Unformatted text preview: BIOREFINERY CONCEPT (“urn Grain Si I‘m: In Corn Slover St ream LCellulose Composition of corn starch 20-25% Amylase (linear) no r m “Iqumsgyflm. ° x m.» w “r ' l a: ' l a l.hr|lnkngn \ M l \‘ 0' no "W M 75-80% Amylopeotin (branched) r w . .o a _ m , . 0,-- u m m. Amylopccnn - Several thousand glucoee Lesrdues per molecule One branch point new 20 -25 glucose residue»: Immobilized Glucose Isomerase System Largest industrially relevant biocatalysis reaction (11 million tons per year (tpy)) Extensive literalure documentation Immobilized and soluble forms available Inexpensive, nontoxic, easily analyzed substrate and product Extremely stable enzyme (I.A @ssuc = 171 days) emu uni-on o 0 on u I,.LlinLc-gu Composition of corn stover NREL supplied com slover lo all projecl participants (source: BloMass AgriProducts‘ Harlan IA) mm _ Slover washed and dried in small commercial operation, knife milled lo pass ‘A inch round screen Arabinan Mannan Umnlc Acid Non—structural Sugars B. Dale - MSU Energy And Global Warming Potential A in .J \ D l— E >- 0 m I.I.| Z IJJ ‘ ”mam i ”ammo lemma momma 7 women s,aaa,aau momma momma co2 Sink . LCA thru Polymer — Comparing Platforms Glucose isomerase: most important enzyme (l?) EC. 5.3.1.5. Tetramer, composed of two dimers Subunit: 43 kDa Mg catalytically essential, also requirement for Co; Mg/Ca-ratio crifical for proper activity Found originally as a xylose isomerase ...
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