Unformatted text preview: Russell & Zinder Cell 50 1077 1987 Studied transformation of oriC plasmids in dam ' cells
0 \M’ hemi—CH3ated plasmid — transforms poorly ’13) camel un- CH3ated plasmid ~ transforms well -i “W'Vs 9 fully CH3ated plasmid — 1 round replication only ~‘P Interpretation: If only one strand of duplex CH3ated at
oriC )does not initiate replication Ogden et al. 1988: hemimethylated oriC DNA binds to a membrane protein
components in dam' cells, not dam+ cells Campbell Kleckner 1990 Experiment: Use synchronized cells to follow oriC'
DNA binding to cell membrane component l4 ...
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- Fall '09