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SCAN0020 - Location of oriC and Ter regions in Cell During...

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Unformatted text preview: Location of oriC and Ter regions in Cell During Cell Cycle 1998-2001: Used method of FISH (fluorescence in situ hybridization) Niki et a1 Genes and Development 14, 212, 2000 “ “ 15, 2031, 2001 Make fluorescently labeled DNA probes; specific to DNA sequences (oriC, ter). Fix cells <2] hybridize probes<Xl fluorescence microscopy . Claims: oriC moves m mid cell after replication initiates t_0_ poles. Ter sites remain near mid cell. CD Wm Km“ ongoing replicnfim: & refoiding ...
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