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Unformatted text preview: Transcription Mechanism Overview of Process 1. RNA P binds to specific DNA promoter sites
, 2. Initiation complex & first 6-10 nts of RNA
3. Conformation change to elongation phase 4. Termination of transcription What degree of specificim is needed to
find/ function at promoter sites? E coli genome: ~ 4290 genes, many grouped in
operons, ~ 1500-2000 promoter sites with
different ‘strengths.’/ 4x106 possible sites E coli RNA Pol merase: isolated in 1960’s M.W. = 4.665 X 105 ~ 7000 copies/cell
Main 01 e tide subunits M.W ene beta’ 95' 1.6x105 rpoC
beta 9 1.55xlO5 rpoB 2 alphas «74 4 x104 rpoA
omega 0) leO4
a sigma factor (70*) 0' 72:104 rpoD ...
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- Fall '09
- HENDRIX
- Genetics
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