{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

SCAN0026 - Transcription Mechanism Overview of Process 1...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Transcription Mechanism Overview of Process 1. RNA P binds to specific DNA promoter sites , 2. Initiation complex & first 6-10 nts of RNA 3. Conformation change to elongation phase 4. Termination of transcription What degree of specificim is needed to find/ function at promoter sites? E coli genome: ~ 4290 genes, many grouped in operons, ~ 1500-2000 promoter sites with different ‘strengths.’/ 4x106 possible sites E coli RNA Pol merase: isolated in 1960’s M.W. = 4.665 X 105 ~ 7000 copies/cell Main 01 e tide subunits M.W ene beta’ 95' 1.6x105 rpoC beta 9 1.55xlO5 rpoB 2 alphas «74 4 x104 rpoA omega 0) leO4 a sigma factor (70*) 0' 72:104 rpoD ...
View Full Document

{[ snackBarMessage ]}