Unformatted text preview: Predictin fldenti in romoters in bacteria from
seguence NOT easy . Use consensus sequence and establish a scoring algorithm,
. neural network algorithm, etc to identify promoters. One Example:
htt ://Www.fruitﬂ .or /se tools/ romoter.html For sequences: http://www.ncbi.nlm.nih.gov/ try: E coli galactose operon promoter Determine bases in promoter site contacted by RNA
Polymerase Chemical Probes and RNA P Protection from Probes ‘ - roach 1: First treat DNA with chemical such that ~
1 nucleotide/molecule effected. Bind RNA P. Separate
complex from unbound DNA. Recover unbound.
Break DNA. Gives nucleotides needed for initial
binding. kNail)“ Approach 2: First bind RNA P to DNA. Treat with
probe s.t. <1 nt/DNA affected. Remove RNA P, break
DNA at unprotected sites compare to unbound DNA treated similarly. Information on nucleotides protected
in open complex. " 10 ...
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- Fall '09