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SCAN0033 - Predictin fldenti in romoters in bacteria from...

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Unformatted text preview: Predictin fldenti in romoters in bacteria from seguence NOT easy . Use consensus sequence and establish a scoring algorithm, . neural network algorithm, etc to identify promoters. One Example: htt ://Www.fruitfl .or /se tools/ romoter.html For sequences: http://www.ncbi.nlm.nih.gov/ try: E coli galactose operon promoter Determine bases in promoter site contacted by RNA Polymerase Chemical Probes and RNA P Protection from Probes ‘ - roach 1: First treat DNA with chemical such that ~ 1 nucleotide/molecule effected. Bind RNA P. Separate complex from unbound DNA. Recover unbound. Break DNA. Gives nucleotides needed for initial binding. kNail)“ Approach 2: First bind RNA P to DNA. Treat with probe s.t. <1 nt/DNA affected. Remove RNA P, break DNA at unprotected sites compare to unbound DNA treated similarly. Information on nucleotides protected in open complex. " 10 ...
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