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Unformatted text preview: Different Sigma Factors —
Different Promoter Consensus Seguences
sigma. & “_-3_5_’ m E
70 general TTGACA l 7 IATAAT
3 2 heat shock CCCTTGAA 13-15 CCCGATNT
5 4 nitrogen CTGGNA 6 TTGCA
F flagellar CTAAA 1 5 GCCGATAA 38 stationary TG(N0_2)C_C_ATAAT* * IBC 2003 Kolb et a1. Transition form Initiation Complex to Elongation Phase Evidence for abortive initiations Hansen and McClure 1980
Studied different DNA promoters on fragments: Transcription assay: Leave out one rNTP, short RNAs made constantly, sigma still bound to core if disrupt Markers prod Prod Add all NTPs: Still see
short RNAs! sigma
released ...
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- Fall '09
- HENDRIX
- Genetics
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