SCAN0047 - ldCEp‘fi we;EE;EE.W‘ ‘ii ’4'” Wm...

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Unformatted text preview: ldCEp‘fi we? ;;;EE;EE.W‘ ‘ii ’4'”; Wm “1““; .. V g“ k, J5 ,1 '3») 35???? EE“ EE; Wm} mam” it. 29% 51E? “* 1940’s and 1950’s: early studies E coli — isolated beta galactosidase Activity: lactose *4 glucose + galactose Monod: for media with little glucose 5010 4010 N 3010 g 2010 1010 10 lactose can induce 500X-1000X increase in bGz . Why & How? Additional confounding facts: 1. Discovery of gratuitous inducer: EMWMfilagggige. Not metabolized yet induces increase in bGz ! i (37H Allnlamnse 2. Observed ‘Catabolite Repression” — IF have glucose then WWW” neither IPTG or lactosemndygeflsmbwggybyilflflgx. Initially _, “wwwfiwm. (fired glucose repression. ...
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