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SCAN0051 - Many more D sites than 0R1 sites ~ 4x106/1...

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Unformatted text preview: Many more D sites than 0R1 sites: ~ 4x106 /1 Ko/Kns = 107 is high enough to allow R to bind 0R1. BUT inducer decreases binding specificity to Karo/K Enables - .. 24: ottom Line: occupation of operator by repressor governed by coupled equilibria. Depends on K values and concentrations of a_ll components. Can Illustrate Use: [R]tot = 10 molecules/10'15 liters [Dns]tot = 4x1 06 sites/10'15 liters [0] tot = l site/10‘15 liters Use: Equilibrium binding equations, constants and assumption 0f [Dns]tot= [Dns] 9 and [Rtot] = [R0] + [RD] + Bk \0 can show inducer changes f from :34;ng ...
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