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img084 - 5 Determine the net electrical charge of the...

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Unformatted text preview: 5. Determine the net electrical charge of the following peptide in solution at pH = 3.7 . , ,.—-——————— r, 1, \1. S, 3, 5* 2 5 +1 @Ala-ArgtAsp-Val - caOH ‘f‘ifi‘iw”’“+‘“w """’ H , ' ' 6) NH; We)“; (all (”00 Free amino acid pKa information {1 PH ; g _ 7; + l. C Alanine;}«3/ 9.7 q. 9 ‘7‘ 3' 1} 2, b Argmme;@%. 12:5 P4,,ct. + I Aspartate;y9,~3,7;,'9/'6/ Q G9 [00“ f o’ Valine'; 2'3’%. Mb ”“1, i O O S N“; (OOH LPI—li'ha' + ' a) +1.50 ‘ or, 3} 9g 3 5} 7 b +1.00 01? I25 3-” ILSZ'q’ A I I! t -71.? 0 0.50 ’ Iii/MRS {9415 ("(1) (no d) 0.00 a e) -0.50 \JJ {”437 — 0. Y Hint: Draw the stick-f1 gure of the polypepti e r each charged structure. ~” 6. What is the isoelectric point for the following polypeptide (same as previous question)? Ala-Arg-Asp-Val Free amino acid pKa information Alanine; 2;){97 5 V P3 — «:2 Arginine; ' ,9.0, 12.5 Aspartate;A/§M, 9.6 Valine;}{, 9.6 \ 72,? a) 2'8 fl»? . *7 Ql‘fi'mp’? ‘174 ‘ V / e) 8.1 i ” 7. How many amino acid residues-per-turn are there in a g-strand? / Q20 b) 3.6 c) 4.4 d) 5.0 ...
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