Unformatted text preview: lau Pinang, Malaysia E-mail: [email protected] The present study aimed to develop and validate a simple reversed -phase high performance liquid chromatographic method with UV detection at 260 nm for the determination of ketoprofen in human plasma. The sample preparation involved a simple protein precipitation method using perchloric acid along with acetonitrile as the deprotenizing agents. Chromatographic separation was achieved isocratically by using C-18 column (5 µm, 250 x 4.6 mm ID). The mobile phase was comprised of 0.05 M disodium hydrogen phosphate buffer and acetonitrile (70:30, v/v) adjusted to pH 6.5 with glacial acetic acid. Analysis was run at a flow rate of 1.0 mL/min with 50 µL injection volume and the samples were quantified using peak height measurement. The calibration curve was linear in the range of 156.25 20000 ng/mL with correlation coefficient (r) of 0.9999 (±0.0001). The within-day accuracy ranged between 83.80 and 112.56 % with precision between 3.41 and 7.07 %. The between -day accuracy ranged between 91.57 and 99.84 % with precision between 1.62 and 16.06 %. The recovery ranged between 96.45 and 102.92 % with precision between 2.90 and 9.92 %, respectively. The values of accuracy, precision and recovery obtained were within the acceptable limits as proposed by USFDA Bioanalytical Guidelines. In conclusion, the developed and validated method can be used to estimate plasma ketoprofen concentration after oral administration of pharmaceutical dosage forms. 76 3rd ICYC 2010 Universiti Sains Malaysia ANA-P2 23rd-25th June 2010 DEVELOPMENT AND VALIDATION OF A LIQUID CHROMATOGRAPHY – TANDEM MASS SPECTROMETRY METHOD FOR DETERMINATION OF ARTEMISININ IN RAT PLASMA Gamal Osmam Elhassan, Yuen Kah Hay, Wong Jia Woei, Samer AL-dahli and Jiayuddin Khan School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia E-mail: [email protected] Artemisinin (ART) or qinghaosu is a naturally occurring sesquiterpene lactone endoperoxide isolated from a traditional Chinese medicinal herb, Artemisia annua L., it is an effective antimalarial drug. The objective of this study was to develop and validate a sensitive and specific LC -MS/MS method for the determination of artemisinin concentration in rat plasma using amolodipine a...
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