12 the retention time of about 39 min is the

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Unformatted text preview: FULLERENE NANOPARTICLES 1,2 Kasra Saeedfar and 1,* Lee Yook Heng 1 School of Chemical Sciences and Food Technology, Faculty of Science and Technology Universit y Kebangsaan Malaysia, 43600, Selangor, Malaysia 2 Chemistry Department, Faculty of Science, K. N. Toosi University, Tehran, Iran E-mail: yhl1000@ukm.my One of the metabolic products of protein is urea. Amount of urea is important in glomerule filtration rate and renal function testing. Urea is widely used in fertilizer as a convenient source of nitrogen. Therefore determination of urea is essential in biomedical applications. The enzyme urease can be employed for the determination of urea, where urease cat alyzes the hydrolysis of urea to form alkaline reaction products. To construct a biosensor, urease that contains an amino group can be immobilized onto fullerenes or carbon nanotubes via modified –COOH group, through the reaction of N,N’dicyclohexylcarbodiimide. Results of Fourier transform infra-red spectroscopy (FTIR), transmission electron microscopy (TEM) and protein assay showed that urease was chemically attached on the surface of these nanoparticles and there was no leaching of enzyme observed. Thes e enzyme bioconjugated nanoparticles were coated onto a screen printed electrode (SPE) deposited with a photocured n-butyl acrylate membrane containing pH sensitive hydrogen ionophore. Thus by using potentiometric method to determine the changes in pH caus ed by urease catalysis of urea reaction, the amount of urea present can be measured. The buffer capacity and pH conditions for the potentiometric -2 biosensor were optimized and the linear response range of the biosensor towards urea was from 10 to -5 10 M and the sensitivity slope (51 mV/decade) was close to Nernstian value. Therefore, carbon nanotube and fullerene nanoparticles have been successfully used for enzyme immobilization for the construction of a potentiometric urea biosensor. ANA-P19 PHOTOLUMINESCENCE STUDIES ON THE BINARY AND TERNARY LANTHANIDE COMPLEXES AR Mazratul-Adzfifi, J NurAsmidar, B Saad and MI Saleh School of Chemical Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia E-mai...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.

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