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Unformatted text preview: -O7 23rd-25th June 2010 OPTIMIZATION OF AQUEOUS ENZYME-ASSISTED HEMPSEED OIL EXTRACTION USING RESPONSE SURFACE METHODOLOGY
1,3 Sajid Latif, Farooq Anwar and Muhammad Arif 1 2 Department of Chemistry and Biochemistry, University of Agriculture Faisalabad 38040, Pakistan 2 Department of Statistics, University of Agriculture Faisalabad -38040, Pakistan 3 Institute for Animal Production in the Tropics and Subtropics (480), Department of Aquaculture Systems and Animal Nutrition, University of Hohenheim, D-70593, Stuttgart, Germany E-mail: [email protected] Response surface methodology (RSM) was employed to optimize enzyme -assisted aqueous extraction (EAAE) of hempseed oil (HSO) using Viscozyme L enzyme. The effects of process parameters: enzyme concentration (0.5 - 2.0 %), time of hydrolysis (1.5 - 2.5 h), water to seed (w/s) ratio (5 - 9) and pH (3 - 7) were appraised. A central composite experimental design (CCRD) was used to ascertain optimised EAAE 2 conditions. The fitted model provided good approximation to the experimental condition (r = 0.99, CV = 0.29 %). The maximum oil yield of 28.9 % (ca. three times higher than the control) was obtained at enzyme concentration=1.75 (seed weight basis), pH = 5, w/s ratio = 7 and hydrolysis time = 2 h. The quality attributes such as iodine value, density, refractive index, unsaponifiable matter, and fatty acid composition of HSO, produced by enzyme adjuvant, were comparable with the control and cold pressed oils revealing no considerable variations (p >0.05). While, lower color values were observed for the enzyme-extracted and control HSO than that of cold pressed oil. The total phenolic content, DPPH• scavenging capacity, inhibition of linoleic acid peroxidation and tocophero ls concentration of the enzyme-extracted oil were found to be higher than those of the control and cold pressed HSO thus indicating superior antioxidant attributes. It was concluded that EAAE can be a good alternative to conventional cold pressing for the simultaneous rec...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.
- Spring '11