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Unformatted text preview: f present investigations it is reasonable to say that methanolic extracts of dry pea’s have exhibited varying degree of antioxidant activity. 64 3rd ICYC 2010 Universiti Sains Malaysia ANA-O3 23rd-25th June 2010 DEVELOPMENT OF MODIFIED ELECTRODES FOR THE MECHANISTIC STUDY OF LIPOXYGENASE AND FATTY ACIDS OF COOKING PALM OIL
1 Zahra A Jarjes, Mohamad Razip Samian and Sulaiman Ab Ghani
2 1 1 2 School of Chemical Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia School of Biological Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia E-mail: [email protected] The hydrolysis of cooking palm oil by a commercial lipase from yeast of Candida rugosa has been studied. The optimum enzyme activity was obtained at pH 7.5, temperature 37 °C, incubation time 60 min, enzyme 0.1 g and substrate weight 2 g. The optimum condition was also studied for the hydrolysis of cooking palm oil on carbon electrodes. The cyclic voltammetry of redox reactions between liberated fatty acids substrate with the lipoxygenase enzyme immobilized on ammonium salts modified nafion membrane on the carbon electrodes were investigated. At medium pH 7 and in potassium phosphate -2 solution as buffer and also as supporting electrolyte a current density of 1.4 mA cm was obtained. This cathodic current was ascribed to the direct reduction of peroxyl radical of fatty acids on the electrodes. ANA-O4 METHOD DEVELOPMENT FOR DETERMINATION PHENOLIC ANTIOXIDANTS IN SEVERAL PERSONAL CARE PRODUCTS USING RP-HPLC-UV/Vis Mohammed Akkbik, Zaini Assim and Fasihuddin Ahmad Department of Chemistry, Faculty of Resource Science & Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak E-mail: [email protected] Simultaneous determinations of phenolic antioxidants, such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and octyl methylcinnamat (OMC) in several personal care products such as sunscreen cream, milk lotion, hair oil and hair gel has been optimized and validated using reversed phase high performance liquid chromatography (RP-HPLC) equipp...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.
- Spring '11