Unformatted text preview: was completely inhibited. NAT-P5 BIOTRANSFORMATION OF PREDNISOLONE AS A TOOL FOR GREEN CHEMISTRY REACTION Zaimi M. N., Sadia S., Syed A. A. S. and Weber J.F.F. Institute for the Study of Natural Remedies (iKUS), Faculty of Pharmacy, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia E-mail: [email protected] Structural modifications of chemical compounds are tools to study the functional changes in the biological activity groups and chemical properties of the molecules. This may be achieved by biotransformation or green chemistry reactions. Prednisolone is co mmonly used in the treatment of severe asthma, rheumatic disorders, renal disorders and diseases of inflammatory bowel, skin, and gastrointestinal tract. In order to obtain further interesting derivatives of bioactive substances, prednisolone was incubated with various microorganisms namely HAB10R12, NW30, HAB11R3, 11L2, HAB2R1 (endophytes from iKUS collection), T. roseum, A. niger, C. ellegens, R. stolonifer and B. cinerea (ATCC strains). Only T. roseum fermentation broth shows evidence of metabolic changes. Two additional peaks were observed on the extract by HPLC chromatogram when compared with the chromatograms of control and substrate and assumed to be transformed products. These transformed products will be further subjected to spectroscopic technique with 1D and 2D-NMR for structure confirmation. 172 3rd ICYC 2010 Universiti Sains Malaysia NAT-P6 23rd-25th June 2010 ANTIOXIDANT ACTIVITY OF SIX SELECTED MALAYSIAN ‘ULAM’ Adlina Adnan, Norizan Ahmat, Nur Ainaa Atikah Mohd Nazri and Sharifah Aminah Syed Mohamad Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor, Malaysia E-mail: [email protected] Six types of ‘ulam’ namely Mentha arvensis (mint), Lactuca sativa var capitata(cabbage lettuce), Psophocarpus tetragonolobus (winged bean), Pluchea indica Less. (beluntas), Premna cordifolia (bebuas) and Limnocharis flava L. (paku rawan) were investigated for their antioxidant activity. Antioxidant activity was determined by free radical scavenging activity (DPPH) assay. Besides, TPC were also having been compared between selected plants. All plants were extracted successively with d...
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