In order to further develop these compounds larger

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: le. The purification of those peptides was carried out using two successive orthogonal separation steps. The first one is a gel filtration process using Preparative HPLC from Japan analytical industry using Polystyren gel column (Polymeric GPC column i.d. 20 X 600 mm) medium pressure, using Chloroform and Tetrahydrofuran as a solvent system with 2.5 mL/min flow rate. The presence of the expected compounds in one of th e fractions was confirmed by LC-MS/MS. The peptides were then fractionated onto an analytical column in HPLC system equipped with a diode-array detector (DAD) and evaporative light scattering detector (ELSD). All analyses and separations were carried out in a reverse phase mode, using a Synergy column ® (150 × 4.6 mm, 4 µm particle size, Phenomenex , USA with flow rate 1 mL/min) with a guard column filled with the same material. The column temperature was maintained at 36 °C. The monitoring of the separation is carried out by both a DAD and an ELSD (as these pe ptides are not much UV-active). A fraction collector connected to the LC system allowed the collection of the pure targeted peptides. Their identity was confirmed by LC-MS/MS and capillary NMR. The additional peptides were also collected and their structure elucidation is in process. 173 23rd-25th June 2010 3rd ICYC 2010 Universiti Sains Malaysia NAT-P8 POTENT ANTIMICROBIAL ACTIVITIES OF SECONDARY METABOLITES OF CURRY LEAF ( MURRAYA KOENIGII L. SPRENG) AGAINST ANTIBIOTIC RESISTANT CLINICAL MICROBES 1 Thilahgavani Nagappan, Perumal Ramasamy and Charles Santhanaraju Vairappan 2 1 Laboratory of Natural Product Chemistry, Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Sepangar Bay, 88999 Kota Kinabalu, Sabah, Malaysia 2 Department of Biochemistry, School of Medicine, Universiti Malaysia Sabah, Se pangar Bay, 88999 Kota Kinabalu, Sabah, Malaysia E-mail: [email protected] Widespread use of antibiotics in clinical medicine is playing a significant role in the emergence of resistant bacteria. The surge of antibiotic resistant microbes cannot be taken li ghtly as they have started to show resistance against vancomycin, which was regarded as the last resort in clinical practice. This has become a strong catalyze in reigniting a frantic search for novel metabolites with potent antimicrobial potential. Hence, we have started the search for antibacterial metaboli...
View Full Document

This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.

Ask a homework question - tutors are online