Methanolic extract showed highest antioxidant

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Unformatted text preview: stry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: Rosalindkim@hotmail.com The main objective of this research is to isolate chemical constituents from stem bark of Goniothalamus tapisoides (Annonaceae). The plant material is extracted with hexane, dichloromethane, and methanol. Each of the crude is examined for their chemical constituents by us ing TLC. After that, the crude extracted is subjected to column chromatography on silica gel, elute with suitable solvent so that the compounds can be separated and purified. The structural elucidation will be carried out on the pure 1 13 compounds by spectroscopic methods such as H NMR, C NMR, COSY, DEPT, HMBC, HSQC, IR and UV. The dried and milled stem bark of G. tapisoides was defatted with hexane for three days and filtered. The plant material is then air dried and moistened with 25 % ammonia solution and left to soak overnight. After that, the plant material is dried again, followed by extraction with methanol by soaking them in large beakers for 3 days. All filtrate is concentrated under reduced pressure to yield the extracted crude. Each of the crude is examined for their chemical constituents by using TLC. After that, the crude extracted is subjected to column chromatography on silica gel, elute with suitable solvent so that the compounds can be separated and purified. The fractions collected are groupe d into a series of fraction after monitoring with TLC. Two compounds have been found and identified from dichloromethane crude of G. tapisoides species, which are goniothalamin and pterodondiol. Goniothalamin is styryl lactones while pterodondiol is sesqui terpenes. Preliminary studies on the dichloromethane extract from the stem barks of G. tapisoides has resulted in the isolation and characterization of two pure compounds which are goniothalamin and pterodondiol. NAT-P24 ISOLATION, IDENTIFICATION AND EVALUATION OF COMPOUNDS EXTRACTED FROM HIBISCUS ROSA SINENSIS LINN. THAT PROMOTING HAIR GROWTH 1 Chiang Pui Nyu...
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