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Unformatted text preview: Maynila, Gen. Luna St., Intramuros 1002, Manila, Philippines E-mail: [email protected] The current paper tested the specificity and sensitivity of the Formalin Ether Concentration Technique against p11/p12 PCR; the gold standard recognized by the CDC in diagnosing Entamoeba histolytica infections. One-hundred ninety stool specimens from 5 government h ospitals in Manila were tested. FECT was performed upon collection. DNA was directly extracted from stool specimens using the freeze thaw method. Primers that amplify a 100 bp E. histolytica novel gene were used to test true positive cases. 90 and 75 stool samples tested positive using FECT and PCR respectively. Out of the microscopy positive samples, only 75 were positive for the p11/p12 gene. Correspondingly, sensitivity and specificity rates were 83.33 and 88.46 %. Computed p value using the Fisher’s exa ct test for sensitivity and specificity were 0.22 and 0.001 respectively. It is mean that the ability of FECT to detect true positive cases was comparable to PCR. However, its ability to determine true negat ive cases was poorer. This mean that many FECT positive samples collected were actually infected with E. dispar. This commensal is a morphologically identical but genetically distinct species of Entamoeba. It was proven that most E. histolytica positive samples were actually infected with E. dispar. In conclusion, in terms of sensitivity, FECT is statistically similar to PCR. With regards to specificity, PCR is still superior to FECT. 239...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.
- Spring '11