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Unformatted text preview: m Salhin School of chemical Sciences, Universiti Sains Malaysia (USM), 11800 Pulau Pinang, Malaysia E-mail: [email protected] A C16H16N2O4 chromogenic Schiff base ligand has been synthesized. Th e ligand structure was 1 13 characterized by CHN elemental analysis, FT-IR, H NMR and C-NMR spectroscopy. The chromogenic complexation behavior of the ligand towards alkali, alkaline earths and transition metal ions has been 2+ investigated. Preliminary finding shows that the ligand is preferentially bind with Cu in a solvent 2+ mixture (water/acetonitrile, v/v). Upon the addition of Cu to an equivalent amount of the ligand in a solvent mixture (water/acetonitrile), a large bathochromic shift with clear change of colour (from colourless to pink) was observed. The experimental parameters that affect the complexation of the 2+ ligand with Cu , such as solvent type and composition, standing time, metal concentration, and ligand concentration are under investigation. 82 3rd ICYC 2010 Universiti Sains Malaysia ANA-P14 23rd-25th June 2010 SEPARATION OF PHENOLIC ACIDS IN HONEY BY CAPILLARY ZONE ELECTROPHORESIS Nur Bahiyah Abu Bakar, Khaldun M. Al Azzam, Ahmad Makahleh and Bahruddin Saad School of Chemical Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia E-mail: [email protected] Honey is rich in phenolic acids, this has attracted lots of attention due to their positive role in contributing to human health and act as natural antioxidants. The simultaneous determination of phenolic acids such as caffeic acid, gallic acid, cinnamic acid, ferulic acid, chlorogenic acid, syringic acid, vanillic acid, benzoic acid, p-hydroxybenzoic acid, 2,4-dihydroxybenzoic acid, o-coumaric acid, mcoumaric acid, p-coumaric acid and vanillin as an internal standard was investi gated by capillary zone electrophoresis (CZE). The analytical procedure involves their extraction from the sample, followed by the analytical separation and quantification. Honey samples were separated by solid -phase extraction through C18 column for sugar removal. The effects of several factors such as pH, concentration of the running buffer, ratio of organic solvents, separation voltage, injection time and the wavelength of UV d...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.
- Spring '11