Unformatted text preview: terials has been widely recognized but only phosphol ipids from soybean and egg that has been used so far. Here we report our study of the preparation and characterization of liposomes from coconut phospholipids (coconut liposomes). In our study the preparation was done by freeze-thawing and followed by extrusion technique. Characterization was done using Cryo-TEM to determined liposomes size and lamellarity while the leakage properties were investigated by fluorescence spectroscopy using carboxyflurescence as fluorescence agent. The coconut liposomes obtained were compared to control liposomes obtained from soybean and egg phospholipids. It was established that coconut liposomes have characteristics and properties more or less similar to control. The liposomes size can be as small as 100 nm. After extrusion t hey all form unilamellar liposomes. The leakage investigation showed that after 2 hours the leakage patterns are similar for the three of them. Coconut liposomes leak more entrapped carboxyflurescence, 40 % compare to soybean and egg liposomes, 10 and 12 % respectively. After addition of 30 % cholesterol the coconut liposomes leakage was reduced to 10 % while the others are 5 and 12 % respectively. The leakage data showed that for coconut liposomes the addition of cholesterol reduce the leakage more effect ively than for other liposomes. The results suggest that the prospect of using coconut phospholipids as liposomes materials is promising. PHY-O8 SIMULATION OF LYOTROPIC GLYCOLIPID BILAYERS IN VARYING WATER CONCENTRATION Vijay Manickam Achari, Thorsten Heidelberg and Rauzah Hashim Department of Chemistry, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: [email protected] Molecular dynamics simulation has been applied to study the stability of glycolipid bilayers and the structure characteristics of surfactants in lamellar phases in the absence of water as well as at varying water concentrations namely low, intermediate and high. The investigation aims on the pre...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.
- Spring '11