The bark is used to treat female infertility and

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Unformatted text preview: ns Malaysia NAT-O11 ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITIES OF MALAYSIAN SEA CUCUMBER, STICHOPUS HERMANII EXTRACTS 1 Osama Y. Althunibat, Ridzwan Hashim, Muhammad Taher and Jamaludin Mohd. Daud 1 2 1 Kulliyyah of Science, International Islamic University Malaysia, Jalan Istana, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia 2 Kulliyyah of Pharmacy, International Islamic University Malaysia, Jalan Istana, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia E-mail: thnibatjust2001@yahoo.com Sea cucumber is a marine invertebrate of the phylum Echinodermata and the class Holothuroidea that found on the sea floor worldwide. In Asia, many of sea cucumber species are considered as traditional food items and have been used in treatment of numerous diseases such as eczema, arthritis and hypertension. Previous scientific studies have shown multiple biological activities of sea cucumber species as antinociceptive, antimicrobial and antifungal properties. This study was conduct ed to investigate the antioxidant and antiproliferative activities of aqueous and organic extracts from sea cucumber Stichopus hermanii. Two different free radical systems were used to evaluate antioxidant capacity of S. hermanii, stable radical 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and linoleic acid free radical mediated β-carotene bleaching. In addition, Folin–Ciocalteau reagent was used to determine the total phenolic contents of the extracts. The inhibitory effects of the extracts on the proliferation of h uman non-small lung carcinoma (A549), cervical cancer cells (C33A) and human breast adenocarcinoma (MCF 7) were demonstrated by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Our data showed that the gallic acid equivalent (GAE) of the total phenolic contents in aqueous extract (GAE = 5.24 mg/g) is higher than an organic extract (GAE = 1.49 mg/g). Both extracts showed low capacities in scavenging of DPPH free radical (IC50 > 10 mg/mL). In contrast, both aqueous and organic extracts exhibited potential antioxidant activities by using β-carotene bleaching assay, giving antioxidant activities equal to 79.62 and 46.66 % respectively. On the other hand, o...
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