Unformatted text preview: e aqueous extracts were also determined by the Folin -Ciocalteau method. The TPC value for the leaves of var intermedia was highest (97.29 mg GAE/100 g extract) compared to other varieties. In the DPPH assay, this variety also showed the highest radical scavenging activity (83.33 %). The results indicated that among the four varieties Ficus deltoidea var intermedia exhibited the strongest antioxidant potential. 179 23rd-25th June 2010 3rd ICYC 2010 Universiti Sains Malaysia NAT-P18 ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF CRUDE AND FRACTIONATED EXTRACT OF ALPINIA PAHANGENSIS
1 Phang Chung Weng, Sri Nurestri Abd Malek, Halijah Ibrahim and Norhanom Abd Wahab 1 1 2 1 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur , Malaysia 2 Institute of Postgraduate Studies, University of Malaya, 50603, Kuala Lumpur, Malaysia E-mail: [email protected] Alpinia pahangensis which belongs to the family Zingiberaceae, is a perennial shrubs and grows wild in East Peninsular of Malaysia. In this study, the total phenolic content was determined whilst the antioxidant activity was assessed using the DPPH radical scavenging a nd the -carotene assays. The cytotoxic activity was determined using spectrophotometric technique, based on the Neutral Red cytotoxicity assay, according to the method of Borenfreund and Puerner and evaluated as IC 50 values. The samples were extracted using methanol and then fractionated with hexane, ethyl acetate and water. The cytotoxic effect of the crude and fractionated extract of Alpinia pahangensis were tested on four cancer cell line namely MCF7 (breast), CaSki (cervical), KB (nasophargeal), HCT -116 (colon) and one normal lung fibroblast cell lines, MRC5. Among the four extracts, the hexane fraction showed the highest cytotoxic activity with IC50 value of 5.8 µg/mL, 21.9 µg/mL, 18.4 µg/mL and 8.9 µg/mL on KB, MCF7, Caski and HCT-116, respectively. In contrast, the hexane fraction showed the lowest antioxidant activities. This is followed by the ethyl acetate fraction showed a moderate cytotoxic effect with IC 50 of 10.2 µg/mL, 50.1 µg/mL, 35.2 µg/mL and 19.9 µg/mL on KB, MCF7, Caski and HCT -116, respectively. However, the ethyl aceta...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.
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