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Unformatted text preview: icrowave digestion prior to the solid samples analysis. Response Surface Methodology (RSM) based on two -level factorial design with four variables was successfully implemented for the optimization study of ultrasonic-assisted enzymatic digestion (USAED) conditions. The interactions and modelled graph between the factors, which are volume of enzyme used (100 - 300 L), time of sonication (30 - 70 mins), weight of sample (0.1 - 0.5 g) and pH of digestion medium (6-10) were explored. Trace metal, Ni was determined using flame atomic absorption spectroscopy (FAAS). The total of 52 experiments were conducted; which give high coefficient of 2 determination (R = 0.9952) of the model. This indicated the suitability of the model for adequately representing the real relationship among the parameters studied. The RSM indicated that enzyme volume of 150 L, sonication time of 30 minutes, sample weight of 0.3 g and pH of 8.7 were the optimum conditions for digestion of TORT-2 reference material and biological samples. 102 3rd ICYC 2010 Universiti Sains Malaysia 23rd-25th June 2010 POSTER PRESENTATION (ENVIRONMENTAL) ENV-P1 UTILIZATION OF FRUITS SKIN AS A BIOSORBENT FOR THE REMOVAL OF METHYLENE BLUE FROM WATER 1,* Siew-Teng Ong, Seok-Theng Ooi, Weng-Nam Lee and Eng-Cheong Khoo 1 2 2 1 Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia 2 Department of Science, Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, Jalan Genting Kelang, Setapak 53300 Kuala Lumpur, Malaysia E-mail: [email protected] The potential of using low cost biosorbents in the removal of basic dyes from aqueous solution has been investigated. The adsorption behaviour of agricultural waste and various fruits skin for Methylene Blue (MB) was carried out as a function of pH, contact time, concentration of MB, amount of adsorbent and sorption isotherms. The sorption process was rapid with high percentage of uptake taking p...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.

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