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Unformatted text preview: ERS AS HOST MOLECULES FOR RECOGNITION OF COENZYMES
1 Banafsheh Gorji, Saeed Taghvaei-Ganjali and Reza Zadmard 1 2 1 Department of Chemistry, Islamic Azad University, South Tehran Branch, Tehran, Iran 2 Chemistry and Chemical Engineering Research Center of Iran, Tehran, Iran E-mail: email@example.com Molecular recognition involves the association of molecules through non-covalent interactions that often called host-guest interactions. These interactions are highly selective, and can be found in many natural complexes such as enzyme-substrate, antibody-antigen. Researchers are concerned about calixarenes with regards to having bowl-shaped molecules and their ability to performing molecular capsules. These capsules can be encapsulated biological guest molecules. Selective recognition of cofactors such as flavin adenine dinucleotide (FAD) due to its role in redox reactions in many biological systems is very important. In this study, a type dimeric-calixarene which can selective bind FAD in aqueous solution was synthesized. In the first part, N,N-bis[23-(5,11,17-tri-tertbutyl-25,26,27,28tetrapropoxy-calixarene)]–adipamide was synthesized. The synthesized dimer was soluble in watermethanol (1:1). In the second part, the study and molecular recognition of biological guests from coenzyme category such as FAD, coenzyme-A and nicotinamide adenine dinucleotide (NADH) were investigated. For this purpose, the synthesized dimer has been used to study thermodynamic and determination of associate constant Ka. Due to the isoalloxazine ring in the structure of FAD which has a strong fluorescence emission, the fluorescence spectroscopy method has been applied for this project. 5 -1 Results prove that Ka for FAD was more than other coenzymes (2.3×10 M ) and the synthesized calixarene is able to recognize FAD selectively. ORG-P11 LIPOPHILIC 2-[(2-CHLOROPHENYL) AND (3-CHLOROPHENYL)] -4-THIAZOLYL -1,4-DIHYDROPYRIDINES: SYNTHESIS, CALCIUM CHANNEL ANTAGONIST ACTIVITY, AND PROTECTION AGAINST PENTYLENETETRAZOLE-INDUCED SEIZURE
1 A. Samzadeh-Kermani, H. Shafaroodi,...
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- Spring '11