Unformatted text preview: es and products frequently contaminated with AFs and Fms include corn, wheat, barley, rice, oats, nuts, milk, cheese, peanuts and cottonseed. Conventional analytical methods for the analysis of these mycotoxins include thin-layer chromatography, high-performance liquid chromatography, gas chromatography, capillary electrophoresis and enzyme linked immune-assay. Most of liquid chromatographic methods employ particulate columns, mainly C18 columns, and have been widely used for the separation of mycotoxins in complex mixtures. However, routine analysis requires high-resolution and faster separations. The more recent monolithic columns that are made via sol–gel technology which enables the formation of highly porous material, (containing macropores and mesopores in its structure) represent an interesting advance in column technology. The unique combination of the very large internal surface area together with significantly higher total porosity result s in a reduction of the diffusion path and provides high permeability. The monoliths thus exhibit significantly lower back -pressures than the conventional columns and allow much higher flow rates to be employed. The main benefit of these high flow rates is the significant reduction in the overall run times, whilst maintaining separation efficiencies that are comparable to those obtainable with 5 µm particulate columns. This presentation will focus on the HPLC method development on the separation of AFs an d Fms using the monolithic column, and their applications for the analysis of these mycotoxins in real samples. ANA-O6 SEPARATION OF CHIRAL COMPOUNDS USING CAPILLARY ELECTROPHORESIS Khaldun M. Al Azzam, *Bahruddin Saad and Rohana Adnan School of Chemical Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia E-mail: [email protected] Capillary electrophoresis (CE) has shown great promise in complementing the conventional separation methods, especially electrophoresis and liquid chromatography. CE is a high resolution separation technique which is a...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.
- Spring '11