Unformatted text preview: s Internal Standard. The method consist a simple liquid-liquid extraction with methyl tertiary butyl ether (MTBE) with subsequent evaporation of the supernatant to dryness followed by the analysis of the reconstituted sample by LC-MS/MS with a Z-spray atmospheric pressure ionization (API) interface in the positive ion multiple reaction monitoring mode was used to monitor precursor product ions of m/z 282.70 m/z 209.0 for artemisinin and m/z 408.9 m/z 237.0 for amolodipine respectively. The method was linear (0.999) over the artemisinin concentration range of 7.8 – 2000 ng/mL in plasma. The method has a lower limit quantification of 3.9 ng/mL for artemisinin in the plasma. The intra - and inter-day accuracy were measured to be within 94 - 104.2 % and precision (CV) were all less than 5 %. The extraction recovery means for internal standard and all artemisinin concentration used were 82 -85 %. The present method was successfully applied to the characterization of the pharmacokinetic profile of artemisinin in rats after an oral administration. ANA-P3 UREA FRACTIONATION OF POLYUNSATURATED FATTY ACID FROM JATROPHA CURCAS SEED OIL Bashar Mudhaffar Abdullah and Jumat Salimon School of Chemical Sciences and Food Technology, Faculty of Sci ence and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia E-mail: [email protected] The selectivity and efficiency of urea complex (UC) formation -based fractionation of free fatty acids (FFA) were examined. A rapid, simple, and inexpensive procedure recently developed for urea fractionation was applied to lipid containing polyunsaturated. Urea treatment proved useful for isolating polyunsaturated FFA (PUFA) from FFA derived from Jatropha curcas seed oil by removal of saturated and monounsaturated FFA. In situations where FFA within the crystalline or UC phase was ric h in PUFA, the urea/FFA mole ratio of the UC was relatively high. The distribution of urea between the crystalli ne phase and the solvent was neither sign...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.
- Spring '11