Com simultaneous determinations of phenolic

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Unformatted text preview: ed with UV/Vis detector. The analysis can be completed less than 6 minutes. An optimum result was achieved using mobile phase consists of Phase A (acetonitrile) and Phase B (mixture of water: acetic acid, 99:1, v/v). The dynamic range was between 1 to 250 mg/L with relative standard deviation below 0.8 %. Limit of detection for BHA, BHT and OMC were 0.78, 0.44 and 1.57 mg/L, respectively. While limit of quantification for BHA, BHT and OMC were 2.37, 1.33 and 4.77 mg/L, respectively. Recovery study was performed by spiking standard antioxidants at four different concentration levels using external standard addition method. The recovery for BHA, BHT and OMC were ranged from 92.5 - 104.8 %, 83.2 - 107.9 % and 87.3 - 103.7 %, respectively. The concentration of BHA in commercial personal care products, namely sunscreen cream, milk lotion, hair oil and hair gel were 4.87, 4.51, 1.27 and 3.91 mg/g, respectively. The amount of BHT in these personal care products were 1.33, 2.67, 0.215 and 0.869 mg/g, respectively. While, the amount of OMC in the same product was 65.9, 14.48, 0.124 and 0.817 mg/g, respectively. The concentration of phenolic antioxidants in these four personal care products were below than maximum allowable range in cosmetic formulation i.e 0.0002 - 0.5 % (w/w) for BHA and BHT and up to 7.5 % (w/w) for OMC. 65 23rd-25th June 2010 3rd ICYC 2010 Universiti Sains Malaysia ANA-O5 ADVANCES IN COLUMN TECHNOLOGY FOR THE ANALYSIS OF AFLATOXINS AND FUMONISINS 1 Wejdan Shakir Khayoon, Bahruddin Saad and Baharuddin Salleh 1 2 1 2 School of Chemical Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia School of Biological Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia E-mail: wijdansh2007@yahoo.com Aflatoxins (AFs) and fumonisins (Fms) are toxic metabolites produced by the fungi (i.e. Aspergillius and Fusarium) that readily colonise crops in the field or after harvest. Many foods and feeds can become contaminated with these mycotoxins since they can form in commodities before harvest, during the time between harvesting and drying, and during storage. Commoditi...
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