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Unformatted text preview: ichloromethane (DCM) and ethanol (EtOH). Winged bean has the highest scavenging activity in both different extracts. The percentage scavenging for DCM and ethanolic extracts of winged bea n were 89.63 and 92.27 % respectively. Total phenolic content (TPC) of each extracts were also determined. Ethanolic extract of Pluchea indica shows highest value of phenolic content. The results show that some ulam possess antioxidant activity and therefore it could be used as a natural preservative ingredient in food and/or pharmaceutical industries. NAT-P7 ISOLATION OF MINOR BIOACTIVE METABOLITES FROM MALAYSIAN ENDOPHYTIC FUNGI
1 Rasha S.A., Sadia S., Cole A.L.J., Kalavathy R., Munro M.H.G., and Weber J.F.F. 1 2 1 3 1 1 Institute for the Study of Natural Remedies (iKUS), Faculty of Pharmacy, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia 2 School of Biological Sciences, University of Canterbury, Christchurch, New Zealand 3 Department of Chemistry, University of Canterbury, Christchurch, New Zealand E-mail: [email protected] Endophytic Aspergillus HAB10R12 was isolated from Garcinia Scortechinii (Kenondong Krot @ Kandis), collected in a secondary forest in Kuala Pilah. It was shown to contai n notably 5 cytotoxic peptides, for which a patent was applied. The five peptides showed excellent activity against human breast cancer, and colonic carcinoma. Two of the peptides A-3 and A-5 were 4000 times more active when compared with commercial anticancer drugs (fluorouracil, cisplatin and tamoxifen). In order to further develop these compounds, larger quantities are needed. Unfortunately, these peptides are produced in a very small quantity together with a number of non active compounds. For this purp ose (100 units of 20-cm Petri dishes) were inoculated and fermented according to a standardized procedure, by batches of 10 plates each. Each batch was extracted with Ethyl acetate based on our standard operation procedure. Each extract was then subjected to LC-MS/MS technology Triple Quadrapo...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.
- Spring '11