Unformatted text preview: A validated HPLC method was developed for the quantification of deferiprone in human plasma using ultraviolet detection. Chromatographic separation of deferiprone in plasma was performed on a C 18 column, with a mobile phase of methanol-buffer (18:82, v/v), adjusted to pH 3.5, and caffeine was used as an internal 2 standard. The calibration curve was linear over the range 0.25 - 10 g/mL in human plasma (r = 0.9994). After oral administration of deferiprone (500 mg) to human, the plasma concentration -time curve of deferiprone was best conformed to two-compartment open model. The deferiprone plasma concentration showed a rapid absorption and the AUC o f deferiprone was 17.0 ±1.23 h µg/mL. Total body clearance, volume of distribution and elimination rate constant of deferiprone after administration of deferiprone were 32.7 ±2.78 L/h, 129 ±32.0 L and 1.21 ±0.47 L/h, respectively. Average absorption and elimination half-life values of deferiprone of 24 volunteers were 0.62 ±0.12 hours and 2.65 ± 0.43 hours. This study confirms the rapid absorption of deferiprone in humans. AUC was similar t o that previously reported but Cmax was slightly lower than that stated in the literature. The data from the study will be used to satisfy the regulatory requirement for marketing of a generic drug in Pakistan. Further studies on large segments in thalasse mia patients of the local population for kinetic analysis are recommended. 68 3rd ICYC 2010 Universiti Sains Malaysia ANA-O11 23rd-25th June 2010 MINERAL COMPOSITION OF STRAWBERRY, MULBERRY AND CHERRY FRUITS AT DIFFERENT RIPENING STAGES Tahir Mahmood, Farooq Anwar and Tahira Iqbal Department of Chemistry & Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan E-mail: [email protected] Mineral contents of strawberry (Fragaria x ananassa Duch), mulberry (Morus alba, M. nigra, M. macrus and M. leavigata) and cherry (Prunus avium L.) fruits at different stages of maturity were analyzed using ICP-OES. The concentrations of macro -minerals (Ca, K, Mg, Na and P) for strawberry fruits (DW) at un ripened, semi-ripened and fully-ripened stages varied from 2300 to...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.
- Spring '11